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Cell Signaling Technology Inc antibodies specific for phospho (p)-dynamin-related protein 1 (drp1) (ser616)
The effect of acetaminophen (APAP) and naringin (Nar) on mitochondrial dynamics. A , The equilibrium of mitochondrial dynamics. APAP promoted mitochondrial fission and Nar promoted mitochondrial fusion. B and C , Western blot analysis of the effects of Nar and APAP on mitochondrial fission <t>(p-DRP1/DRP1).</t> D , E , and F , Western blot analysis of the effect of Nar and APAP on mitochondrial fusion (OPA, Mfn 1). Data are reported as means±SD (n=3). **P<0.01 and ***P<0.001 vs the APAP group (ANOVA).
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The effect of acetaminophen (APAP) and naringin (Nar) on mitochondrial dynamics. A , The equilibrium of mitochondrial dynamics. APAP promoted mitochondrial fission and Nar promoted mitochondrial fusion. B and C , Western blot analysis of the effects of Nar and APAP on mitochondrial fission (p-DRP1/DRP1). D , E , and F , Western blot analysis of the effect of Nar and APAP on mitochondrial fusion (OPA, Mfn 1). Data are reported as means±SD (n=3). **P<0.01 and ***P<0.001 vs the APAP group (ANOVA).

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Naringin regulates mitochondrial dynamics to protect against acetaminophen-induced hepatotoxicity by activating the AMPK/Nrf2 signaling pathway in vitro

doi: 10.1590/1414-431X2022e12040

Figure Lengend Snippet: The effect of acetaminophen (APAP) and naringin (Nar) on mitochondrial dynamics. A , The equilibrium of mitochondrial dynamics. APAP promoted mitochondrial fission and Nar promoted mitochondrial fusion. B and C , Western blot analysis of the effects of Nar and APAP on mitochondrial fission (p-DRP1/DRP1). D , E , and F , Western blot analysis of the effect of Nar and APAP on mitochondrial fusion (OPA, Mfn 1). Data are reported as means±SD (n=3). **P<0.01 and ***P<0.001 vs the APAP group (ANOVA).

Article Snippet: Antibodies specific for mitofusin protein 1 (Mfn1), optic atrophy type 1 (Opa1), phospho (p)-dynamin-related protein 1 (Drp1) (Ser616), total Drp1, AMPK, p-AMPK (Thr172), Nrf2, and GAPDH were purchased from Cell Signaling Technology (USA).

Techniques: Western Blot